Figure 3.

Sequence alignment of Moritella DHFRs sorted from lowest to highest P_G. Unique residues are indicated by triangles for MpDHFR (blue), and MyDHFR (yellow). Secondary structure elements are indicated by horizontal bars: α-helices (gray), β-strands (black) and the Met20 (yellow), CD (blue), FG (green) and GH (magenta) loops. M. japonica DHFR left out due to low sequence identity (28.85%) to MpDHFR. Sequence numbering is based on E. coli DHFR, with the insertion in Moritella DHFRs between residues 65 and 66 of E. coli DHFR denoted as 66.5.