Figure 1.

The EAAT promoter shows stronger activity than the CYP27A1 5′ UTR in some cell lines and in vivo

(A) Schematic representation of luciferase reporter plasmids containing the CMV promoter, the hybrid liver-specific promoter EAAT (albumin enhancer linked to the α1 anti-trypsin promoter), and the 5′ UTR from the human CYP27A1 gene (C27P). The promoter-less plasmid pGL3-Basic was used as a negative control. (B) The plasmids were transfected in the indicated liver-derived cell lines from human (HuH-7, HepG2, and Hep3B) and mouse (Hepa1-6 and AML12) origin. Luciferase activity was measured in cell extracts obtained 48 h after transfection. The result is expressed as percentage of activity, considering the CMV promoter as a reference. (C) The plasmids were injected in C57BL/6 mice (n = 6) by hydrodynamic injection, and light emission was quantified by BLI 48 h later. Bars represent means ± SEM for each group. ∗p < 0.05, Kruskal-Wallis.