Figure 7.
AAV8-EAAT-CYP27A1, but not CDCA, normalizes bile acid composition in blood of CTX mice
The AAV8-EAAT-CYP27A1 vector was administered intravenously to 7-week-old CTX mice at the indicated doses (×1012 vg/kg). CDCA was mixed in mouse chow at 0.5%. Untreated CTX and WT littermates were included as a reference. Blood was collected 5 months after the initiation of treatment, and the main free bile acids and tauroconjugates were analyzed in plasma. Bars represent means ± SEM for each group. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Kruskal-Wallis with Dunn’s post test. aMCA, α-muricholic acid; bMCA, β-muricholic acid; CA, cholic acid; CDCA, chenodeoxycholic acid; DCA, deoxycholic acid; HDCA, hyodeoxycholic acid; LCA, lithocholic acid; TCA, taurocholic acid; TCDCA, taurochenodeoxycholic acid; TDCA, taurodeoxycholic acid; THDCA, taurohyodeoxycholic acid; TLCA, taurolithocholic acid; TMCA, tauromuricholic acid; TUDCA, tauroursodeoxycholic acid; UDCA, ursodeoxycholic acid.