Fig. 4.

Cell viability and Wnt/β-catenin signal intensity in response to IC-2. (a) Cell viability by WST assay in various concentrations of IC-2 (n = 3). Mean ± SD, ^∗P < 0.05, and ^∗∗P < 0.01 compared with 0.1% DMSO, examined by Dunnett's test. (b) Wnt/β-catenin signal intensity in response to various concentrations of IC-2 by reporter assay (n = 3). Mean ± SD, ^∗P < 0.05, and ^∗∗P < 0.01 compared with 0.1% DMSO, examined by Dunnett's test. Relative luciferase activity was expressed as corrected firefly luciferase activity with the internal control renilla luciferase activity calculated against 0.1% DMSO.