Figure 1.

Establishment of the human duodenal organoid-derived monolayer

(A) Image of the duodenal lumen obtained during upper gastrointestinal endoscopy. A biopsy from the non-inflammatory lamina propria mucosae of the duodenum was performed with the patient’s consent. (B) Collected specimens were in the low protein adsorption tube during the crypt isolation process. (C) Bright-field images of the isolated crypts (left panel) and established organoids (center panel, day 10 of establishment; right panel, day 5 of passage) are shown.