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. 2021 May 19;22:263–278. doi: 10.1016/j.omtm.2021.05.005

Figure 3.

Figure 3

Gene expression levels in the human duodenal organoid-derived monolayer

The gene expression profiles in the human duodenal organoid-derived monolayer (organoid-monolayer), Caco-2 cells (Caco-2), and adult duodenum (total RNA was purchased from the BioChain Institute) were characterized. The organoid monolayer at 8 days after seeding is shown. (A) Gene expression levels of CYP enzymes (CYP2C19, CYP2C9, CYP2J2, CYP2S1, CYP3A4). (B) Gene expression levels of CES enzymes (CES1, CES2). (C) Gene expression levels of UGT enzymes (UGT1A1, UGT2B17, UGT2B7). (D) Gene expression levels of transporters expressed on the apical side of the human small intestinal epithelium (BCRP, MCT1, MDR1, PEPT1). (E) Gene expression levels of transporters expressed on the basolateral side of the human small intestinal epithelium (MRP3, OCT1). Data are expressed as means ± SD (n = 3). The expression level of GAPDH in each group was taken as 1.0. Statistical significance was evaluated by one-way ANOVA followed by a Tukey’s post hoc test. Groups that do not share the same letter had significantly different results (p < 0.05).