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. 2021 Jul 22;13(8):2499. doi: 10.3390/nu13082499

Figure 2.

Figure 2

Impact of chronic leptin treatment on body temperature and BAT UCP1 expression in LD mice. (a) Body temperature (°C), (b) BAT mUcp1 mRNA expression determined by RT-qPCR, (c) UCP1 protein expression determined by Western blot, and (d) BAT mPgc1a, (e) BAT mCidea, and (f) BAT mDio2 mRNA expression determined by RT-qPCR of control mice, saline-treated LD mice, and 3.0 mg/kg BW/d leptin-treated LD mice. Data are presented as mean ± SEM and represent N ≥ 4 per group. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc test. *: indicates p < 0.05; ***: p < 0.001; ****: p < 0.0001.