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. 2021 Jul 29;9(8):1621. doi: 10.3390/microorganisms9081621

Figure 2.

Figure 2

(A) The number of rhoptry kinases ROPK expressed in each developmental stage for EtINRAE, according to the putative classes of kinase activity (non-canonical in green, inactive in red, and active in black). Gene expression was standardized with the expression level in gametes: genes with a fold change > 2 were considered as transcribed. Two-way ANOVA, different letters (a, b, c) refer to statistical differences between the groups (p < 0.05). “a” means that the number of ROPK expressed is statistically different between NSO and all other stages, “b” means that the number of ROPK expressed is not statistically different between SO and eSPZ stages, but it is statistically different in comparison with all other stages, “c” means that the number of ROPK expressed is not statistically different between iSPZ, ImmSCHZ, MatSCHZ, MI, and MII stages, but it is statistically different in comparison with NSO, SO, and eSPZ. (B) The expression level of ropk was quantified in each developmental stage for EtINRAE (black) and EtWIS (grey) strains, expressed as log fold change (FC) in comparison with the gamete stages. Data are expressed as the mean ± SD of two independent experiments. Two-way ANOVA showed no significant difference between strains and between developmental stages.