Table 4.
Primers used in conventional PCR/nested PCR to confirm microfluidic real-time PCR results.
| Pathogen | Targeted Gene | Primers | Sequence (5′-3′) | Amplicon Size (bp) | Reference |
|---|---|---|---|---|---|
| Rickettsia spp. | gltA | Rsfg877 Rsfg1258 |
GGGGGCCTGCTCACGGCGG ATTGCAAAAAGTACAGTGAACA |
381 | [157] |
| ompB | Rc.rglt.4362p Rc.rompB.4836n |
GTCAGCGTTACTTCTTCGATGC CCGTACTCCATCTTAGCATCAG |
475 | [158] | |
| Rc.rompB.4496p Rc.rompB.4762n |
CCAATGGCAGGACTTAGCTACT AGGCTGGCTGATACACGGAGTAA |
267 | |||
| Anaplasma/Ehrlichia spp. | 16S rRNA | EHR1 EHR2 EHR3 |
GAACGAACGCTGGCGGCAAGC AGTA(T/C)CG(A/G)ACCAGATAGCCGC TGCATAGGAATCTACCTAGTAG |
629 | [159] |
| Hepatozoon spp. | 18S rRNA | HepF HepR |
ATACATGAGCAAAATCTCAAC CTTATTATTCCATGCTGCAG |
660 | [160] |
| Theileria spp. | 18S rRNA | BABGF2 BABGR2 |
GYYTTGTAATTGGAATGATGG CCAAAGACTTTGATTTCTCTC |
559 | [161] |
| BTH 18S 1st F BTH 18S 1st |
GTGAAACTGCGAATGGCTCATTACR AAGTGATAAGGTTCACAAAACTTCCC |
1500 bp | [162] | ||
| BTH 18S 2nd F BTH 18S 2nd R |
GGCTCATTACAACAGTTATAGTTTATTTG CGGTCCGAATAATTCACCGGAT |
1500 | |||
| Coxiella burnetii and Coxiella-like endosymbionts | 16S rRNA | Cox 16SF1 Cox 16SR1 |
CGTAGGAATCTACCTTRTAGWGG ACTYYCCAACAGCTAGTTCTCA |
719–813 | [52] |
| Cox 16SF2 Cox 16SR2 |
TGAGAACTAGCTGTTGGRRAGT GCCTACCCGCTTCTGGTACAATT |
625 | |||
| Borrelia spp. | flaB | FlaB280F FlaRL |
GCAGTTCARTCAGGTAACGG GCAATCATAGCCATTGCAGATTGT |
645 | [163] |
| FlaB737F FlaLL |
GCATCAACTGTRGTTGTAACATTAACAGG ACATATTCAGATGCAGACAGAGGT |
407 | |||
| Bartonella spp. | gltA | bart781 bart1137 |
GGG GAC CAG CTC ATG GTG G AAT GCA AAA AGA ACA GTA AAC A |
380–400 | [164] |