Figure 4.

Effect of ACE-inhibitors and AT₁r antagonists on Caco-2 cell barrier integrity. Caco-2 cell barrier was preincubated at day 18 with ACE-inhibitors, AT₁R antagonists, or vehicle (DMSO) in the indicated concentrations for 72 h, infected with SARS-CoV-2 (MOI 0.1) and incubated for the indicated time points. The cell barrier integrity was determined by measurement of the TEER value. The TEER values obtained after 24 h, 48 h, and 72 h were normalized to the TEER value obtained at 0 h. The experiment was carried out in three biological replicates. For statistical analysis, one-way ANOVA with multiple comparisons test was used. * p < 0.01, ** p < 0.01, **** p < 0.0001 show statistically significant differences between drug-treated and vehicle-treated samples at the same time point.