Figure 2.
Expression of HERV-K env protein and levels of HERV-K env mRNAs in PBMC of ALS patients and controls. The protein expression was evaluated by Flow cytometry of HERV-K env protein in PBMC subpopulations of ALS patients and HCs; in particular: (a) in B cells CD19; (b) in Natural killer cells CD56/16; (c) in monocytes CD14; (d) Detection of HERV-K env mRNAs in PBMCs of ALS patients and HCs, by real-time RT-PCR (means of three experiments run in duplicate, calculated by the 2−ΔCt methods). RT-PCR: reverse transcription-polymerase chain reaction. PBMC: peripheral blood mononuclear cell; and ALS: amyotrophic lateral sclerosis.