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. 2021 Aug 18;7(8):669. doi: 10.3390/jof7080669

Figure 8.

Figure 8

RAxML tree based on analysis of combined LSU, SSU, ITS and TEF1-a dataset. The combined analyses include 47 strains with 3464 characters including gaps (LSU: 943 bp, SSU: 1036 bp, ITS: 528 bp and TEF1-a: 957 bp). The tree is rooted to Sporidesmium cangshanense (MFLUCC 15–0420) and S. dulongense (MFLUCC 17–0116). Tree topology of the maximum likelihood analysis Bayesian analysis are similar. The RAxML analysis of the combined dataset yielded a best scoring tree (Figure 8) with a final ML likelihood value of −17099.345044. The matrix had 1090 distinct alignment patterns, with 48.94% undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.241037, C = 0.253983, G = 0.288692, T = 0.216288; substitution rates AC = 1.213787, AG = 2.314464, AT = 1.495483, CG = 1.069500, CT = 6.672100, GT = 1.000000; gamma distribution shape parameter α = 0.250596. Bootstrap values for ML ≥ 75% and Bayesian posterior probabilities (PP) ≥ 0.95 are labelled on the nodes. The ex-type strains are in bold and black. The newly obtained sequence is indicated in red.