Figure 16.

RAxML tree based on analysis of combined LSU, SSU, ITS and RPB2 dataset. The combined analyses include 74 strains with 4212 characters including gaps (LSU: 1136 bp, SSU: 1139 bp, ITS: 564 bp and RPB2: 1144 bp). The tree is rooted to Ceratostomella pyrenaica (CBS 129343) and C. cuspidata (CMP 17629). Tree topology of the maximum likelihood analysis and Bayesian analysis are similar. The RAxML analysis of the combined dataset yielded a best scoring tree (Figure 16) with a final ML likelihood value of −39,690.726824. The matrix had 1778 distinct alignment patterns, with 30.92% undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.248100, C = 0.243558, G = 0.284201, T = 0.224142; substitution rates AC = 1.409308, AG = 3.086772, AT = 1.346045, CG = 1.235614, CT = 5.843915, GT = 1.000000; gamma distribution shape parameter α = 0.200153. Bootstrap values for maximum likelihood (ML) ≥ 75% and Bayesian posterior probabilities (PP) ≥ 0.95 are labelled on the nodes. The newly obtained sequence is indicated in red.