Figure 2.

Experimental plan. Bone fragments and peripheral blood samples derived from the same osteoporotic patient were used as sources of osteoblasts (hOBs) and osteoclast precursors (human monocytes, hMCs), respectively. hOBs were treated or not with MK-4 (10 μM) for 14 days, both in a 2-D conventional culture system (upper part of the scheme) and 3-D co-culture with hMCs producing 3-D bone cells aggregates (lower part of the scheme).