Figure 5.

Protective activity of different EVs preparation and of recombinant SerpinB3. (A) Histograms represent the percent of gain of survival of HepG2 cells treated with 250 μM H₂O₂ for 72 h in presence of different EVs or of recombinant SerpinB3, compared to untreated cells. SB3 concentration was identical to that detected in the corresponding EVs-SB3 preparations. (B) Histograms represent the percent of gain survival in cardiomyocytes treated with 250 μM H₂O₂ in presence of different EVs preparations at 1.00 × 10⁷/mL concentration or of recombinant SerpinB3, compared to untreated cells. Both experiments were performed in triplicate. (C) Fluorescence images of Live and Dead assay in primary cardiomyocyte treated for 2 h with 250 μM H₂O₂ in presence of different EVs preparations or of recombinant SerpinB3. Original magnification 63×.