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. 2021 Aug 3;14(8):759. doi: 10.3390/ph14080759

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of the hepatitis C virus (HCV) replicon in cell assays. Evaluation of the potency and cytotoxicity of temperature-inactivated Tyr50 kDa in cell (Huh-5-2) assays for (A) partially inactivated at 37 °C and (B) completely inactivated at 80 °C Tyr50 kDa. HCV replicon replication rate (luminescence, black bars) and cell survival (closed squares) were independently measured in cell culture by increasing protein concentration to determine EC50. * All luminescence and viability values were normalized considering the untreated control values (UTC).