Table 4.
Analysis of chronic toxicity of formulation L in C57Bl/6 mice. Complete blood count of animals after four intravenous (IV) administrations of liposomal formulation L or saline buffer.
| Blood Parameter | Day 0 1 | OptiMEM | L | ||||
|---|---|---|---|---|---|---|---|
| 1 TD 2 | 2 TD 2 | ||||||
| Day 21 | Day 60 | Day 21 | Day 60 | Day 21 | Day 60 | ||
| Leukocytes × 109/L | 2.5 ± 0.2 | 3.9 ± 0.1 | 3.0 ± 0.3 | 3.1 ± 0.2 | 2.7 ± 0.4 | 2.6 ± 0.3 | 2.4 ± 0.2 |
| Lymphocytes × 109/L | 2.2 ± 0.2 | 2.9 ± 0.3 | 2.5 ± 0.2 | 2.6 ± 0.2 | 2.4 ± 0.4 | 2.2 ± 0.2 | 1.9 ± 0.2 |
| Monocytes × 109/L | 0.2 ± 0.1 | 0.5 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| Granulocytes × 109/L | 0.2 ± 0.1 | 0.5 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| Erythrocytes × 1012/L | 9.7 ± 0.3 | 9.0 ± 0.2 | 8.6 ± 0.2 | 9.4 ± 0.2 | 8.7 ± 0.5 | 9.0 ± 0.2 | 8.8 ± 0.2 |
| Thrombocytes × 109/L | 514 ± 94.8 | 833 ± 113 | 757 ± 45.9 | 776 ± 43.6 | 735 ± 74.9 | 920 ± 39.9 | 757 ± 52.3 |
| Hemoglobin g/L | 128 ± 4.1 | 107 ± 3.4 | 107 ± 3.1 | 113 ± 2.9 | 105 ± 5.9 | 111 ± 3.1 | 115 ± 3.7 |
1 Day 0 indicates the initial levels of blood parameters before treatment; 2 TD, therapeutic dose; 1 TD corresponds to 1.5 nmol/g of liposomes.