Table 2.
Polyphenol profile of E. dendroides flower (FE), leaf (LE), fruit (FrE), and branch (BE) extracts. Results were expressed as the mean ± standard deviation of three independent experiments in triplicate (n = 3).
| n a | Compound | RT b | λmax (nm) | [M-H]- | MW c | FE | LE | FrE | BE |
|---|---|---|---|---|---|---|---|---|---|
| g/100 g DE d | |||||||||
| 1 | Gallic Acid | 6.97 | 232;270 | 169 | 170 | 0.08 ± 0.01 * | 0.01 ± 0.00 § | 0.04 ± 0.00 | 0.04 ± 0.00 |
| 2 | 3,4 Dihydroxybenzoic acid | 13.08 | 232 | 153 | 154 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.01 ± 0.00 |
| 3 | p-Hydroxybenzoic acid | 23.97 | 232; 260 | 137 | 138 | 0.03 ± 0.00 * | - | 0.01 ± 0.00 | 0.01 ± 0.00 |
| 4 | Catechin | 26.95 | 279 | 289 | 290 | - | 1.03 ± 0.01 | - | - |
| 5 | Chlorogenic acid | 32.40 | 290; 320 | 353 | 354 | 13.80 ± 0.58 * | 11.66 ± 0.37 | 11.67 ± 0.42 | 0.74 ± 0.01 ° |
| 6 | Caffeic acid | 34.02 | 290; 325 | 179 | 180 | 0.02 ± 0.00 * | 0.17 ± 0.01 § | 0.01 ± 0.00 & | 0.04 ± 0.00 |
| 7 | Epicatechin | 38.23 | 279 | 289 | 290 | - | 0.01 ± 0.00 $ | 0.12 ± 0.01 | - |
| 8 | p-Cumaric acid | 44.38 | 280; 300 | 163 | 164 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.02 ± 0.00 Ψ | 0.01 ± 0.00 |
| 9 | Eriodictyol-7-O-glucoside | 48.31 | 232; 284 | 449 | 450 | 0.08 ± 0.00 * | 0.04 ± 0.00 § | 0.05 ± 0.00 & | 0.06 ± 0.00 |
| 10 | m-Cumaric acid | 50.30 | 230; 274 | 163 | 164 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.01 ± 0.00 | 0.01 ± 0.00 |
| 11 | Verbascoside | 53.86 | 230; 330 | 623 | 624 | - | 0.01 ± 0.00 | - | - |
| 12 | Isovitexin | 54.91 | 284;328 | 431 | 432 | 0.02 ± 0.00 & | - | - | 8.02 ± 0.22 |
| 13 | Isoquercetin | 56.37 | 262; 356 | 463 | 464 | - | - | - | 0.01 ± 0.00 |
| 14 | Rutin | 57.25 | 256;356 | 609 | 610 | 2.76 ± 0.11 * | 0.65 ± 0.04 & | 0.59 ± 0.03 & | 0.05 ± 0.00 |
| 15 | Roifolin | 59.97 | 264; 336 | 577 | 578 | - | - | 0.01 ± 0.00 | - |
| 16 | Kaempferol-3-O-rutinoside | 60.95 | 266; 348 | 593 | 594 | - | - | 0.01 ± 0.00 | - |
| 17 | Isorhamnetin-3-O-rutinoside | 62.55 | 255; 356 | 623 | 624 | 0.41 ± 0.02 * | 0.17 ± 0.01 § | 0.04 ± 0.00 & | 0.01 ± 0.00 |
| 18 | Luteolin | 70.77 | 268; 350 | 285 | 286 | - | - | 0.01 ± 0.00 | - |
| 19 | Tiliroside | 72.24 | 266; 314 | 593 | 594 | - | - | 0.01 ± 0.00 | - |
| 20 | Isorhamnetin | 75.71 | 230; 372 | 315 | 316 | - | - | - | 0.01 ± 0.00 |
| 21 | Apigenin | 75.96 | 267; 336 | 269 | 270 | - | - | 0.01 ± 0.00 | - |
| Total | 17.23 | 13.78 | 12.62 | 9.02 | |||||
a n. = peak number based on the elution order; b RT= retention time; c MW= Molecular weight; d DE= dry extract; * p < 0.001 vs. LE, FrE and BE; § p < 0.001 vs. FrE and BE; ° p < 0.001 vs. LE and FrE; & p < 0.001 vs. BE; $ p < 0.001 vs. FrE; Ψ p < 0.001 vs. FE, LE and BE.