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. 2021 Jul 28;9(8):1611. doi: 10.3390/microorganisms9081611

Figure 4.

Figure 4

Effects of LN12 CFS with different treatments on the H. pylori 3192 biofilm. Heat-treated CFS: LN12 CFS 115 °C heated for 20 min; neutralized CFS: LN12 CFS adjusted the pH to 6.5 with 1 M NaOH; proteinase K-treated CFS: LN12 CFS treated with 1 mg/mL proteinase K (final concentration) for 1 h at 37 °C and inactivated by heating at 95 °C for 1 min; catalase-treated CFS: LN12 CFS treated with 0.5 mg/mL catalase (final concentration) for 1 h at 37 °C and inactivated by heating at 95 °C for 30 min; mMRS: modified de Man Rogosa Sharpe broth; BB2 were the controls. The experimental data are represented by the mean ± standard deviation (n = 3), and the groups marked with different superscript letters indicate statistically significant differences (p < 0.05).