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TCFβ1 is phosphorylated in vitro in an activation-dependent manner. Full-length TCFβ1 (residues 1 to 301) expressed as a GST fusion protein was incubated with cell extracts from Jurkat cells activated with PMA (5 ng/ml), PMA (5 ng/ml) plus PHA (5 μg/ml), or UV for the indicated lengths of time, processed for in vitro kinase assay, analyzed by SDS-PAGE, and visualized by autoradiography. GST alone was used as a negative control.
