TABLE 1.

Conservation of sequences between JNK binding sites in c-Jun and TCFβ1^a

Motif	Protein	Sequence
A	TCFβ1	130 S.KPHTPSLDED 140
	c-Jun	48 SLKPHLRAKNSD 59
	v-Jun	........NNAD
Conserved sequence		S-KPH------D
B	TCFβ1	67 PVAVRKPSTPE.SP 79
	TCFβ1	103 PAPAAKPSASAPIP 116
	c-Jun	51 PHLRAKNSDLLTSP 64
	c-Jun	31 P.KILKQSMTLNLA 43
	v-Jun	.....NNADILTSP
Conserved sequence		P----K-S----SP
C	TCFβ1	128 LVSKPHTPSLDED 140
	TCFβ1	38 LQVQAVTPQLLLN 50
	c-Jun	33 ILKQSMTLNLADP 45
	v-Jun	................
Conserved sequence		LV----T--L-D-

^aSequence comparisons between the JNK binding sites in c-Jun (amino acids 20 to 80) and the activation domain of TCFβ1 (amino acids 1 to 145) reveal a modest degree of sequence conservation. The residue 20 to 80 region of c-Jun was used as a comparison since c-Jun_1–223, c-Jun_1–144, and c-Jun_1–79 bind JNK activity whereas c-Jun_43–223 and v-Jun do not bind JNK activity. The symbol “.” denotes a deletion, whereas the symbol “-” suggests the absence of sequence conservation. The consensus conserved sequence is shown at the bottom of each motif. The numbers denote the amino acid numbers in the native TCFβ1 and c-Jun proteins.