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. 2021 Jul 30;11(8):498. doi: 10.3390/metabo11080498

Figure 2.

Figure 2

Effects of lipid fractions from the culture medium of treated astrocytes on ERK1/2 activity in neuronal cultures. The lipid fractions from astrocytes treated with LPS, ML355, LPS + ML355, Zileuton, and LPS + Zileuton were added to the primary rat cortex neuron cultures for 4 h. pERK1/2 and ERK1/2 protein levels were evaluated by Western blotting and normalized to the loading control β-actin. (A) Results, expressed as fold-changes, relative to the control. (B) Representative Western blots demonstrating phospho-ERK1/2, total ERK1/2, and β-actin protein levels. The example is representative of four independent experiments. The values represent mean ± SD from four independent experiments. *—p < 0.05, compared with the neurons treated with lipid fraction obtained from native astrocytes; #—p < 0.05, compared with the neurons treated with lipid fraction obtained from LPS-stimulated astrocytes.