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. 2021 Aug 2;14(8):757. doi: 10.3390/ph14080757

Figure 3.

Figure 3

Live cell imaging of mutant PfFNT G107S-GFP stably expressed in HEK293 cells and binding of labeled ligands BH296-DY647 and BH267.meta-DY647. BH267.meta-DY647 (b), but not BH296-DY647 (a) co-localizes with the membrane-bound G107S mutant of PfFNT-GFP, and competition with 100 µM BH267.meta prevents membrane localization (c). Based on fluorescence intensity levels, the interaction appears to be weaker than for the wild type PfFNT-GFP to BH296-DY647 (see Figure 1b,c). (d) The interaction cannot compete with BH296. (e) BH267.meta-DY647 localizes with PfFNT wildtype. Incomplete overlap of both channels in E does not represent altered localization but can be attributed to an offset between both channels and thus is irrelevant for the drawn conclusion of the experiment.