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Non-CF Sm airway secretory cells express ATP6V0D2 on the apical plasma membrane. (A–C) Immunostaining of cultured Sm airway epithelial cells for expression of ATP6V0D2 (green), acetylated α-tubulin (red), F-actin (white), and DAPI (blue) shows that V0D2 is absent from ciliated cells. (D–F) Immunostaining of cultured Sm airway epithelial cells for expression for V0D2 (green), Muc5B (red), F-actin (white), and DAPI (blue) shows that V0D2 is expressed in Muc5B+ cells. Scale bars, 20 μm.
