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Localization of V-type ATPase is pH-dependent in non-CF Sm airway epithelia. (A and B) When the ASL pH is artificially set at 6.8, the V-type ATPase localizes to the cytoplasm of Sm airway epithelial cells. (C and D) When the ASL pH is set to 7.4, the V-type ATPase translocates to the apical membrane. Quantification of total ATP6V0 d2+ cells (E). Scale bars: A and C, 40 μm; B and D, 20 μm. *P < 0.05 by paired t test.
