Figure 5.

RNA-sequencing data for genes involved in ciliary development and assembly in COPD HBEC and normal HBEC after 0 and 7 days of differentiation on COPD bronchial scaffolds or normal bronchial scaffolds (n = 9 repopulated scaffolds per combination and time point) or at the ALI (n = 3 HBEC donors). Day 0 was defined as the day of differentiation induction, which was 4 days after the seeding of cells. DRC1 (dynein regulatory complex subunit 1) expression was higher in COPD HBEC than in normal HBEC on normal scaffolds on Day 0. No induction was seen between Day 0 and Day 7 for any of the genes in HBEC grown at the ALI, whereas all genes increased significantly over time in normal HBEC grown on bronchial scaffolds. In COPD HBEC grown on bronchial scaffolds, the following genes increased between Day 0 and Day 7: ZMYND10, ARMC4, and RSPH1 (both scaffold types) and DRC1, DNAI2, and TMEM231 (normal scaffolds only). Means and SDs are indicated. *FDR < 0.05, **FDR < 0.01, and ***FDR < 0.001 for COPD HBEC compared with normal HBEC grown on bronchial scaffolds using DESeq2 (see the data supplement) and the Benjamini-Hochberg method for multiple testing correction. ARMC4 = armadillo repeat–containing 4; B9D1 = B9 domain–containing 1; CC2D2A = coiled-coil and C2 domain–containing 2A; DNAI2 = dynein axonemal intermediate chain 2; RSPH1 = radial spoke head component 1; TMEM231 = transmembrane protein 231; ZMYND10 = zinc finger MYND type–containing 10.