Figure 1.

Leptin-induced Trpm7 (TRP [transient receptor potential] melastatin 7) promoter activity in rat adrenal pheochromocytoma (PC12) cells expressing LEPRb (long, active form of leptin receptor) (PC12^LEPRb cells). PC12^LEPRb cells were transfected with pEZX-Luc-Trpm7 (a Luc [luciferase] reporter of Trpm7) or a control (CTL) vector (pEZX-Luc [an empty promoter Luc reporter]) followed by treatment with leptin at 1, 10, or 100 ng/ml for 3 days. Luc activity was examined. There was no change in luminescence in the PC12^LEPRb cells transfected with CTL vector upon leptin treatment (data not shown). All data are represented as a ratio of relative luminescence units (RLU) of the pEZX-Luc-Trpm7 to the RLU of the CTL vector pEZX-Luc and are shown as mean ± SEM values. An ordinary one-way ANOVA with Tukey multiple comparisons was used to determine the significant differences between groups. *P < 0.05.