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. 2021 Mar 30;65(2):214–221. doi: 10.1165/rcmb.2020-0374OC

Figure 4.

Figure 4.

Leptin modulated H3K4M3, H3K27Ac, H3K27M3, and binding of DNMT3B (DNA methyltransferase 3B) at the Trpm7 promoter. PC12LEPRb cells were treated with 1 ng/ml leptin and/or 5 μM SD1008 for 3 days. Epigenetic modifications at (A) R1, (B) R2, and (C) R3 of the Trpm7 promoter were examined by using ChIP-qPCR. Results are shown as the percent change in enrichment as compared with the PC12LEPRb CTLs and are presented as mean ± SEM values. A one-way ANOVA with Tukey multiple comparisons was used to determine the significant differences between groups. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. H3 = histone 3; H3K4M3 = trimethylation of H3K4; H3K27Ac = acetylation of H3K27; H3K27M3 = trimethylation of H3K27; K4 = lysine 4; K27 = lysine 27.