Figure 6.

(Page 15). Unsupervised hierarchical cluster analysis based on alterations in the release of soluble mediators by primary AML cells derived from 72 unselected patients after treatment with 5 µM chloroquine (CQ) for 48 h. Primary AML cells were cultured with 5 µM chloroquine or medium alone for 48 h, before the levels of soluble mediators in harvested supernatants were determined using Luminex analysis. A hierarchical cluster analysis was performed based upon the alteration of soluble mediators compared to untreated controls (control set to 100%). The cluster analysis identified three patient subsets: (i) a subset with increased release of soluble mediators after treatment with chloroquine (upper subset, shown as dark green in first column to the right), (ii) one intermediate subset, with divergent release of soluble mediators (middle subset, shown as turquoise in column to the right), and (iii) a subset of patients with decreased release of soluble mediators after treatment with chloroquine (light turquoise). There is one outlier at the bottom of the cluster with a very high release of all soluble mediators after treatment with chloroquine (marked in dark green). The columns to the far right indicating proliferative responses and chloroquine effects are based on Figure 4.