FIG. 6.

Transcriptional repression by LexA-Nrg1. The CYC1-lacZ reporters in this experiment were pLG669Z (no lexA operator) and JK1621 (four lexA operators) (48). LexA1-87 DNA-binding domain, LexA-Nrg1 (here designated Nrs1), or LexA-Mig1 was expressed in either wild-type (wt) or mutant strains. Cells were grown in synthetic media lacking uracil and histidine and containing either 2% glucose (repressed condition) or 2% glycerol plus 2% ethanol (derepressed condition). β-Galactosidase activity values are averages of duplicated experiments using three independent transformants.