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. 2021 Aug 19;14(16):4674. doi: 10.3390/ma14164674

Figure 1.

Figure 1

The gel retardation of nanocomplexes. (A) Gel retardation assay of pDNA and peptide nanocomplexes. Nanoparticles were complexed by pDNA and HALA series peptides and RALA peptide. N:P ratios 0–12 were tested. The nanocomplexes were loaded into a 1% agarose gel which was subsequently run within an electrophoresis field of 120 V for 30 min. (B) Gel retardation assay of siRNA and peptide nanocomplexes. Nanoparticles were complexed by non-target siRNA and HALA series peptides and RALA peptide. N:P ratios 0–4, 6, 8, 10, 12 were tested. The nanocomplexes were loaded into a 20% polyacrylamide gel which was run in an electrophoresis field of 80 V for 120 min.