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. 2021 Aug 23;11(8):562. doi: 10.3390/metabo11080562

Figure 7.

Figure 7

In MDA-MB-231 cells, tunicamycin-induced ER stress inhibited EMT via decreasing the content of TβRI in lipid rafts. (A) MDA-MB-231 cells were treated with various concentration of tunicamycin (0, 0.1, 0.25, 0.5, 1, 2 μmol/L). Viability of MDA-MB-231 cells. (B) Expression levels of GRP78 and CHOP. (C) MDA-MB-231 cells were treated with or without tunicamycin. Expression levels of GRP78 and CHOP. (D) Expression levels of TβRI, p-Smad2, and Smad2. (E) Expression levels of E-cadherin and Vimentin. (F) Confocal microscope image for immunofluorescence staining of TβRI and lipid rafts. Magnification, ×400. Analyses for the content of CTxB and TβRI colocalization. The green fluorescence showed the lipid rafts, red fluorescence showed the TβRI. Scale bars represent 40 μm. The concentration of tunicamycin in (C–F) is 1 μmol/L. Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01 versus the control group.