Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 16;26(16):4949. doi: 10.3390/molecules26164949

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Experimental procedures of 3D Cell culture model implementation (CSM and ASM) using a 96-pillar/well plate. (A) Cell and Matrigel mixture dispensed on the surface of the 96-pillar plate. (B) 3D cell and Matrigel mixture directly gelling after dispensing step. (C) Cell down and aggregate in the core of Matrigel at 4 °C before gelling the Matrigel (D) 3D cell and Matrigel gelation step with the pillar surface facing downward. (E) Additional gelling step for 10 min. (F) One-day incubation for stabilizing the cells by stamping the 96-pillar plate into the 96-well plate filled with cell culture medium. (G) Drug exposure by replacing the 96-well plate with another filled with six different drugs. (H) Green fluorescence image scanning after 3D live-cell staining. (I) Representative images for spread 3D cells in the CSM and aggregated 3D cells in the ASM.