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4T1 murine mammary carcinoma cells after 40 min incubation with the PLGA-Cy5.5/DiI nanoparticles: maximum intensity projection of a z-stack (Laser Scanning Confocal Microscopy, Nikon A1 MP). (A)—merged image and differential interference contrast (DIC); (B)—cell nuclei (Hoechst). (C)—DiI fluorescence; (D)—PLGA-Cy5.5 fluorescence; €—Scatterplot representing colocalization between DiI and Cy5.5 fluorescent signals. (F)—Merged image. Scale bar 10 μm.
