FIG. 5.

Tetrahymena H2A/H4 nucleosomal HAT activity facilitates HIV-1 transcription from a chromatin template. An HIV dinucleosome-5S array fragment (10 ng), assembled into an array of 12 nucleosomes, was incubated with acetyl-CoA (1 mM) and peak II, yEsa1p, NuA4, and peak I HAT activities as indicated, and the reaction mixtures were assayed for transcription. GAL4-AH stimulated transcription of a nonnucleosomal G5-E4 DNA was included to serve as a control for RNA recovery. The positions in the gel of the HIV-1 and E4 transcripts are indicated.