Figure 2.

Isolation of Exosomes: exosomes are commonly isolated from the conditioned media. Some common preprocessing steps are required for both the techniques, including collecting conditioned media from MSCs, performing a centrifugation round at 2000× g for 30 min to remove debris. Furthermore, the conditioned media can be subject to any of the two techniques including, Ultracentrifugation (1) or Kit-based methods (2) for isolation of exosomes. These exosomes can be further used for characterization, aliquoting, and storage for future experiments.