Figure 3.

Genetic structure of hemizygous plant T0 and after self-pollination of the resulting progeny T1. Transformed plants regenerated from callus, or after in planta transformation of germinating seedlings, are termed T0 plants. T0 plants are always hemizygous, meaning that there is a copy of the transgene at a novel locus in the plant genome; the DNA gets integrated into one chromosome, but not the homolog at the same locus. T0 plants produce T1 seeds, which in turn develop into T1 plants that carry the transgene in either a hemizygous, homozygous positive, or homozygous negative state in an expected 2:1:1 Mendelian ratio if there is a single copy insertion of the transgene.