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. 2021 Jul 27;13(8):1152. doi: 10.3390/pharmaceutics13081152

Figure 5.

Figure 5

Modulating role of RAW 264.7 macrophages on the action of ion release from MBG-75S on differentiation of MC3T3-E1 pre-osteoblasts. Macrophages cultured in DMEM were indirectly treated with 1 mg/mL MBG-75S deposited on transwell inserts for 24 h, and the supernatants from these cultures were collected and diluted 1:1 with α-MEM. Control RAW 264.7 macrophages were cultured in parallel without material. Subsequently, MC3T3-E1 pre-osteoblasts were treated for 10 days with these conditioned media. Alkaline phosphatase activity (ALP) was evaluated as a specific marker of in vitro osteogenesis after 10 days of culture in the following conditions: MC3T3 CTRL = control pre-osteoblasts cultured in the absence of material, MC3T3 RAW = pre-osteoblasts cultured with medium of control RAW 264.7 macrophages in the absence of material, MC3T3 MBG T = pre-osteoblasts cultured in the presence of 1 mg/mL of MBG-75S deposited on transwell inserts, and MC3T3 RAW MBG T = pre-osteoblasts cultured with medium of RAW 264.7 macrophages previously treated with 1 mg/mL of MBG-75S deposited on transwell inserts. Statistical significance: *** p < 0.005 (comparison with MC3T3 CTRL); ### p < 0.005 (comparison with MC3T3 MBG T).