Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Preparation of conditioned media of RAW 264.7 macrophages and treatment of MC3T3-E1 pre-osteoblasts. RAW 264.7 macrophages were indirectly treated with 1 mg/mL MBG-75S, deposited on 6-well transwell inserts, which is covered by DMEM (A), after 24 h of culture (B), and the media from these macrophage cultures were collected and diluted 1:1 with α-MEM for the subsequent treatment of MC3T3 -E1 pre-osteoblasts for 10 days (C).
