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Characterization of the wild-type (WT) and mutant strain sr22. The WT and sr22 cells were cultured on an S medium at 30 °C for nine days in a large-scale culture. Symbols: closed circles, WT; closed squares, sr22. (A) Cell concentration (cells/mL), (B) amount of TAG per cell (mg/108 cells), (C) TAG concentration (g/L), (D) amount of TAG per dry cell mass (%), and (E) glucose concentration (g/L). The data are indicated as the mean ± SEM of the three independent experiments.
