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Effects of 1–5 on LC3-II and p62 expression levels. HepG2 cells were treated with the indicated concentrations of 1–5 for 24 h (A), or different durations (B). The expression levels of LC3B (A), p62 (B), and β-actin were determined by Western blotting. BBR (50 μM) was a positive control. The data shown are representative of three independent treatments using the same parameters with similar results. (Original Western Blotting Figure see Supplementary Materials).
