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. 2021 Jul 23;12(8):865. doi: 10.3390/mi12080865

Figure 3.

Figure 3

Effects of the printing process on calcium influx triggered by capsaicin. Calcium flux (AF, showed as heatmap of f340/f380; (G), showed as time–response curve of f340/f380) in cultured (AC) and printed (DF) DRG neurons after exposure to vehicle (A,D), capsaicin (100 nM; B,E) and KCl (40 mM; C,F). The maximum amplitude of response evoked by capsaicin and KCl (H). Percentage of capsaicin-responsive neurons (I). Results consist of the means ± S.E.M. Two-tailed unpaired Student t-test. Significant differences are indicated by asterisks (p < 0.05 = *; ns: not significant as p > 0.05). Amplitude represents the point in which the change in the ratio f340/f380 is maximal. Scale bar = 100 µM (AF).