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ROS generation in HT-29 cells after treated with various concentrations of melatonin for 72 h. The ROS generation was measured by DCFH-DA staining. Each bar represents mean ± SEM of three independent experiments. One-way ANOVA, followed by Dunnett’s post hoc test, was performed to compare each treatment group with the control. Statistically significantly different from the control: *, p < 0.05; #, p < 0.01.
