Figure 4.

Western blot analysis of the ER stress in HT-29 cells after treated with various concentrations of melatonin for 72 h. (A) Expression levels of ER stress-related proteins were measured by Western blot analysis. Beta-actin (β-actin) served as a loading control. (B) Bar chart of the protein expressions; each bar represents a mean ± SEM of three independent experiments. One-way ANOVA, followed by Dunnett’s post hoc test, was performed to compare each treatment group with control; statistically significantly different from control: *, p < 0.05; #, p < 0.01.