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. 2021 Aug 20;26(16):5038. doi: 10.3390/molecules26165038

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western blot analysis of the melatonin-treated HT-29 cells. Effects of melatonin on autophagy pathway protein expressions in HT-29 cells after treated with various concentrations of melatonin for 72 h. (A) Expression levels of autophagy proteins were assessed by western blot analysis. (B) Bar chart of proteins expression; each bar represents a mean ± SEM of three independent experiments. One-way ANOVA was performed to compare each treatment group with control; statistically significantly different from control: *, p < 0.05.