FIG. 7.

Mobility shift of the trypsinized remodeled mononucleosome (Tryp. Nuc.). (A) Remodeling reactions (5 μl) were performed for 55 min in the presence (+) or absence (−) of ATP and hSWI-SNF before competing bound hSWI-SNF from the nucleosome with plasmid DNA. After a 15-min incubation with competitor, the reaction mixtures were loaded on a 4.5% acrylamide gel run in 0.5× TBE. Lane 4 shows the hSWI-SNF modified form of the tailless mononucleosomes (Novel). (B) Same as panel A, except that either tailed (lane 1) or tailless (lane 2) mononucleosomes were incubated with ATP and hSWI-SNF before separating on a 5% acrylamide gel run in 1× TBE.