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Cholinergic differentiation immunocytochemistry of NPCN+ cells derived from MSC-WJ. (A) MSC-WJ labeling using the anti-ChAT antibody (FITC). (B) MSC-WJ labeling using the anti-ßIII-TUBULIN antibody (FITC). (C,D) represent the negative controls (MSC-WJ not differentiated) for both markers anti-ChAT and anti-ßIII-TUBULIN, respectively Hoechst was used for labeling the nuclei (Image obtained using an inverted fluorescence microscope ×100, Axio Vert A1, Carl Zeiss, Oberkochen, Germany).
