Scheme 1.

Neurospheres until cholinergic-neurons differentiation. The MSC-UC were seeded on in NFBX to obtained neurospheres. Neurospheres were than mechanical dissociated to obtain the through the neurospheres the neural precursor cells. The neural precursor cells were induced to cholinergic-like neurons through the addition of NGF, bFGF, EGF, and B27 in cultivation media. Abbreviations: MSC-UC: Umbilical cord mesenchymal stem cells; NFBX: Natural Functional Biopolymer Matrix; DMEM: Dulbecco’s Modified Eagle Medium; FBS: fetal bovine serum; P/S: penicillin and streptomycin, NGF: nerve growth factor; EGF: epidermal growth factor, bFGF: basic fibroblast growth factor, B27: neuronal supplementation.