Figure 3.

Concentration of malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) in skin homogenates. Homogenates were incubated in the presence of FeSO₄ used in one of six different concentrations (2400, 120, 600, 300, 150 and 75 µM) plus H₂O₂ (5 mM) and, additionally, in the presence of IPA (0.0, 7.0, 5.0, 2.5, 1.0, 0.5, 0.25, 0.1, 0.01, 0.001 and 0.0001 mM). Data are expressed as the amount of MDA + 4-HDA (nmol) per mg of protein. Bars represent the mean ± SE of three independent experiments run in duplicate. * p < 0.05 vs. the respective concentration of Fe²⁺ (black bars). All bars representing either Fe²⁺ (black bars) or Fe²⁺ plus IPA (gray bars) are significantly higher than bars representing control (white bars).