Figure 5.

Effect of the WSC extract on MAPK signaling and nuclear translocation of NF-κB in LPS-treated RAW264.7 cells. The cells were pretreated with the indicated concentrations of the WSC extract for 3 h and then treated with 1 µg/mL of LPS for 30 min: (a) The protein levels of total and phosphorylated p38, JNK and ERK were determined via western blotting assays; (b) The protein levels of IκB-α and NF-κB p65 in cytosolic and nuclear fractions were determined via western blotting assays; (c) Localizations of nuclei and NF-κB p65 were visualized following immunofluorescence staining.